NGS


Whole genome sequencing means reading and analyzing the whole genome at one time, which provides the most comprehensive information for genetic variation.
Theragen completed the first domestic human genome map and successfully deciphered the genes of various fauna/flora such as whales and tigers.

Service overview

WGRS
  • SNV/Indels
  • Structure Variantion
  • Copy Number Variantion
  • Trio Analysis
WG de novo
  • Genome Assembly
  • Genome Annotation

Main Capture Kit

  • TruSeq Nano DNA Sample Prep Kit
  • Nextra Mate Pair Sample Prep Kit

Sequencing Platform

HiSeq2500 / HiSeq4000 / NovaSeq6000
Although the exome area accounts for only 1% (30 Mb) of the whole genome, it is very important in terms of biological functions.
Exome sequencing is a technology that selectively checks the exome area and is very economical compared to whole genome sequencing.
It is being mainly used in clinical research because it can be effectively applied in gene identification related to cancer and habitual diseases.

Service overview

  • SNV/Indels
  • Copy Number Variantion
  • Trio Analysis

Main Capture Kit

  • SureSelect XT All Exon V5 Kit
  • SureSelect XT All Exon V6 Kit
  • SureSelect XT custom Teir 1

Sequencing Platform

HiSeq2500 / HiSeq4000 / NovaSeq6000
With transcriptome sequencing, differences in the transcriptome expression value of each sample can be checked.
All living things undergo process of transcription of DNA sequences into an intermediate called mRNA to generate protein based on their own genetic information.
If such mRNA is analyzed, you can get information on the genes that have been activated at certain points.

Service overview

Reference Based
  • Gene Expression
  • DEG
  • Splicing Variants
  • Gene Fusions
de novo Based
  • Transcript Assembly
  • Gene Expression
  • DEG
Small-RNA Seq
  • miRNA Expression
  • Identification of miRNA
  • Target Prediction

Main Capture Kit

  • TruSeq Stranded mRNA Sample Prep Kit
  • TruSeq Stranded Total RNA Sample Prep Kit
  • NEXTflex Small RNA-Seq Kit v3

Sequencing Platform

HiSeq2500 / HiSeq4000 / NovaSeq6000
Single-cell transcriptome sequencing identifies diversity among cells and can be applied to various research fields such as screening of cell therapy products, stem cell research, development of anticancer drugs, and basic research on structure development.
The single cells produced with NGS technology have a combination of characteristics of existing data and characteristics derived from amplification skill, and various procedures and methods need to be used to analyze the data.
If single cells are used, various biological interpretations are possible through analyses within cell types, between cell types, and between tissues, which are obtained by clustering analysis of transcriptome genome data.

Service overview

  • Gene Expression
  • Cell Type Signature
  • Tumor Heterogeneity
  • Tumor Microenvironment
  • Single Cell Immune Profiling

Main Capture Kit

Nextera DNA Library Prep Kit

Sequencing Platform

HiSeq4000 / NovaSeq6000
This method selectively checks and analyzes only the desired area using a custom or pre-made kit.

Service overview

BRCAInside / CancerInside

Main Capture Kit

Agilent
Metagenome sequencing is a method to check the genome assembly of all microorganisms that exist in a specific natural environment.
It enables the checking of the microorganism cluster genome profiling from an environmental specimen, its functional significance, and differences in microorganism cluster patterns from other environmental specimens.
As there are increasing reports that human intestinal and oral microorganism cluster patterns are associated with diseases, this analysis technique is expected to be used in a variety of future medical applications.

Service overview

  • 16s rRNA gene PCR
  • Taxonomy Profiling
  • Calculation of OTU
  • Whole Metagenome
  • Whole Metagenome Assembly
  • Taxonomy Profiling
  • Functional Annotation

Main Capture Kit

  • Nextera XT Index Kit
  • TruSeq Nano DNA Sample Prep Kit

Sequencing Platform

MiSeq / HiSeq X / NovaSeq6000